F4/80 Polyclonal antibody

F4/80 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (2)

Applications

IHC, IF/ICC, IF-P, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 28463-1-AP

Print datasheet

Synonyms

F4 80, Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1



经过测试的应用

Positive IHC detected inmouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, mouse liver tissue
Positive IF/ICC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28463-1-AP targets F4/80 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen F4/80 fusion protein Ag28252 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Synonyms F4 80, Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1
Calculated Molecular Weight 102 kDa
GenBank Accession NumberNM_010130
Gene Symbol F4/80
Gene ID (NCBI) 13733
RRIDAB_2881149
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ61549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
IHC protocol for F4/80 antibody 28463-1-APDownload protocol
IF protocol for F4/80 antibody 28463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Bioact Mater

Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis

Authors - Wei Zhang
mouseIF

Nat Commun

Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway

Authors - Yanan Zhang
mouseIF

Redox Biol

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury

Authors - Jing Yu
mouseFC

Biomaterials

Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.

Authors - Haoran Wang
pigIF

Biomaterials

A myogenic niche with a proper mechanical stress environment improves abdominal wall muscle repair by modulating immunity and preventing fibrosis.

Authors - Shengyu Wang
mouseIHC

Adv Healthc Mater

Silk Acid as an Implantable Biomaterial for Tissue Regeneration

Authors - Ziyang Sun