• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated F4/80 Polyclonal antibody

F4/80 Polyclonal Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-28463

Print datasheet

Synonyms

DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3

验证数据展示

  • IF Staining of mouse spleen using CL488-28463

    IF Staining of mouse spleen using CL488-28463

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 F4/80 antibody (CL488-28463) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of RAW 264.7 using CL488-28463

    FC experiment of RAW 264.7 using CL488-28463

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Mouse F4/80 (CL488-28463) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse spleen using CL488-28463

    IF Staining of mouse spleen using CL488-28463

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 F4/80 antibody (CL488-28463) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inmouse spleen tissue
Positive FC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28463-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28463 targets F4/80 in IF, FC (Intra) applications and shows reactivity with Mouse samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Mouse
Immunogen F4/80 fusion protein Ag28252 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Synonyms DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3
Calculated Molecular Weight 102 kDa
GenBank Accession NumberNM_010130
Gene Symbol Emr1
Gene ID (NCBI) 13733
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ61549
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 F4/80 antibody CL488-28463Download protocol
Standard Protocols
Click here to view our Standard Protocols