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Fibronectin Monoclonal antibody
Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (3)
Conjugate
Unconjugated
111
CloneNo.
1G10F9
验证数据展示
经过测试的应用
Positive WB detected in | human plasma tissue, human plasma, rat serum, HuH-7 cells, HepG2 cells, MG-63 cells, U2OS cells |
Positive IP detected in | human plasma tissue |
Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue |
Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66042-1-Ig targets Fibronectin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, Cell treatment |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Fibronectin fusion protein Ag8016 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | fibronectin 1 |
Synonyms | FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG |
Calculated Molecular Weight | 2386 aa, 263 kDa |
Observed Molecular Weight | 263 kDa |
GenBank Accession Number | BC005858 |
Gene Symbol | Fibronectin |
Gene ID (NCBI) | 2335 |
RRID | AB_11182385 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P02751 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
IHC protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
IF protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
IP protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int J Biol Macromol A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects | ||
Biomed Pharmacother Nintedanib prevents TGF-β2-induced epithelial-mesenchymal transition in retinal pigment epithelial cells | ||
Oxid Med Cell Longev HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis | ||
Front Immunol Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis | ||
Ecotoxicol Environ Saf Alleviative effect of melatonin against the nephrotoxicity induced by cadmium exposure through regulating renal oxidative stress, inflammatory reaction, and fibrosis in a mouse model | ||
Environ Pollut Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway. |