验证数据展示
经过测试的应用
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66486-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
CL594-66486 targets G3BP1 in IHC, IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IHC |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Immunogen | G3BP1 fusion protein Ag3728 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Synonyms | G3BP, G3BP 1, G3BP1, HDH VIII |
| Calculated Molecular Weight | 466 aa, 52 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC006997 |
| Gene Symbol | G3BP1 |
| Gene ID (NCBI) | 10146 |
| RRID | AB_2883591 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13283 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 G3BP1 antibody CL594-66486 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Cell Cardiol RBM20S639G mutation is a high genetic risk factor for premature death through RNA-protein condensates. | ||
