TIM3 Monoclonal antibody

TIM3 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C4G3

Cat No : 60355-1-Ig

Print datasheet

Synonyms

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3



经过测试的应用

Positive WB detected inDaudi cells, PC-3 cells, pig thymus tissue, ROS1728 cells, human heart tissue, RAW 264.7 cells, rat heart tissue
Positive FC detected inDaudi cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60355-1-Ig targets TIM3 in WB, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, pig
Immunogen TIM3 fusion protein Ag16901 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hepatitis A virus cellular receptor 2
Synonyms CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 33 kDa, 50-70 kDa
GenBank Accession NumberBC020843
Gene Symbol HAVCR2
Gene ID (NCBI) 84868
RRIDAB_2881464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

实验方案

Product Specific Protocols
WB protocol for TIM3 antibody 60355-1-IgDownload protocol
FC protocol for TIM3 antibody 60355-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

EBioMedicine

Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes

Authors - Fan Wu
humanWB

Oncogene

Tumor cell-intrinsic Tim-3 promotes liver cancer via NF-κB/IL-6/STAT3 axis.

Authors - Hualin Zhang
human

J Pathol

Molecular subtyping reveals immune alterations in IDH wild-type lower-grade diffuse glioma.

Authors - Fan Wu
human

Front Cell Dev Biol

Aberrantly Expressed Galectin-9 Is Involved in the Immunopathogenesis of Anti-MDA5-Positive Dermatomyositis-Associated Interstitial Lung Disease.

Authors - Lin Liang
pig

Front Immunol

Up-Regulation of Immune Checkpoints in the Thymus of PRRSV-1-Infected Piglets in a Virulence-Dependent Fashion.

Authors - Inés Ruedas-Torres
humanWB

Front Immunol

Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.

Authors - Guanzhang Li