• Featured Product
  • KD/KO Validated

IDH1 Polyclonal antibody

IDH1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12332-1-AP

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Synonyms

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD



经过测试的应用

Positive WB detected inHEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12332-1-AP targets IDH1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, sheep
Immunogen IDH1 fusion protein Ag2986 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Synonyms IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
RRIDAB_2123159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

实验方案

Product Specific Protocols
WB protocol for IDH1 antibody 12332-1-APDownload protocol
IF protocol for IDH1 antibody 12332-1-APDownload protocol
IP protocol for IDH1 antibody 12332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

α-Ketoglutarate links p53 to cell fate during tumour suppression.

Authors - John P Morris
  • KD Validated
humanWB,IHC

Nature

IDH mutation impairs histone demethylation and results in a block to cell differentiation.

Authors - Lu Chao C
humanWB

Nature

Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.

Authors - Dang Lenny L
humanWB

Nature

Quantitative flux analysis reveals folate-dependent NADPH production.

Authors - Jing Fan
humanWB

Cancer Cell

Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.

Authors - Maria E Figueroa
humanWB

Cell Stem Cell

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Authors - Zhexin Zhu