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Integrin Alpha 3 Monoclonal antibody
Integrin Alpha 3 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig, mouse and More (1)
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3F9G4
验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, A431 cells, A549 cells, HepG2 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | PC-3 cells, A549 cells |
Planning an IHC experiment? We recommend our IHCeasy ITGA3 Ready-To-Use IHC Kit. ITGA3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66070-1-Ig targets Integrin Alpha 3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, pig, mouse samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | human, pig, mouse |
| Cited Reactivity | human, mouse, rabbit, pig |
| Immunogen | Integrin Alpha 3 fusion protein Ag17293 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) |
| Synonyms | CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a |
| Calculated Molecular Weight | 1066 aa, 119 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC150190 |
| Gene Symbol | ITGA3 |
| Gene ID (NCBI) | 3675 |
| RRID | AB_11042765 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26006 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Alpha 3 antibody 66070-1-Ig | Download protocol |
| IHC protocol for Integrin Alpha 3 antibody 66070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Hypoxia-induced changes to integrin alpha 3 glycosylation facilitate invasion in epidermoid carcinoma cell line A431. | ||
CNS Neurosci Ther Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia | ||
Thyroid Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study. | ||
Front Mol Biosci Golgi Phosphoprotein 3 Promotes Colon Cancer Cell Metastasis Through STAT3 and Integrin α3 Pathways. | ||
Cell Adh Migr Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions. | ||
Mol Hum Reprod Angiopoietin 2 stimulates trophoblast invasion via a mechanism associated with JNK signaling. |
