MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

Cat No : 18008-1-AP

Print datasheet

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

验证数据展示

FC experiment of Ramos using 18008-1-AP
1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (18008-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis using 18008-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 18008-1-AP
IP result of anti-MME,CD10 (IP:18008-1-AP, 3ug; Detection:18008-1-AP 1:500) with Raji cells lysate 2400ug.
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD10 antibody included
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD10 antibody included
IF Staining of human kidney using 18008-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human kidney using 18008-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 18008-1-AP

Size

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经过测试的应用

Positive WB detected in	mouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IP detected in	Raji cells
Positive IHC detected in	human kidney tissue, human renal cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	human kidney tissue
Positive FC detected in	Ramos cells

Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 14 publications below
IHC	See 4 publications below
IF	See 1 publications below

产品信息

18008-1-AP targets MME,CD10 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IF, IHC
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Immunogen	MME,CD10 fusion protein Ag12506 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	membrane metallo-endopeptidase
Synonyms	Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11
Calculated Molecular Weight	80aa,9 kDa; 750aa,85 kDa
Observed Molecular Weight	100 kDa
GenBank Accession Number	BC106070
Gene Symbol	MME
Gene ID (NCBI)	4311
RRID	AB_2146541
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08473
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

实验方案

Product Specific Protocols
WB protocol for MME,CD10 antibody 18008-1-AP	Download protocol
IHC protocol for MME,CD10 antibody 18008-1-AP	Download protocol
IF protocol for MME,CD10 antibody 18008-1-AP	Download protocol
IP protocol for MME,CD10 antibody 18008-1-AP	Download protocol
FC protocol for MME,CD10 antibody 18008-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression Authors - Rui Zhou View Article
human	WB	Brain MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation. Authors - Bing-Lin Zhu View Article
human	WB	Int J Biol Macromol A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin. Authors - Hui Zeng View Article
mouse	WB	Mol Neurobiol Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression. Authors - Yasuteru Inoue View Article
human	IHC	BMC Genomics Genetic control of renal tumorigenesis by the mouse Rtm1 locus. Authors - Jensen José Ricardo JR View Article
mouse	WB	J Alzheimers Dis Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity. Authors - Ling-Li Lv View Article

MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

FC experiment of Ramos using 18008-1-AP

WB analysis using 18008-1-AP

IP experiment of Raji using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IF Staining of human kidney using 18008-1-AP

IF Staining of human kidney using 18008-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Genetic control of renal tumorigenesis by the mouse Rtm1 locus.

Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity.

相关产品

Flow Cytometry Perm Buffer (10X)