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MYH10 Polyclonal antibody

MYH10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21403-1-AP

Print datasheet

Synonyms

Myosin 10, NMMHC-B, NMMHC II-b, Myosin-10, Myosin heavy chain, non-muscle IIb

验证数据展示

  • WB analysis using 21403-1-AP

    WB analysis using 21403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21403-1-AP (MYH10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21403-1-AP

    IF Staining of HepG2 using 21403-1-AP

    Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 21403-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse brain using 21403-1-AP

    IP experiment of mouse brain using 21403-1-AP

    IP result of anti-MYH10 (IP:21403-1-AP, 4ug; Detection:21403-1-AP 1:1500) with mouse brain tissue lysate 3440ug.

  • FC experiment of HepG2 using 21403-1-AP

    FC experiment of HepG2 using 21403-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MYH10 Polyclonal antibody (21403-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21403-1-AP targets MYH10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, bovine
Immunogen MYH10 fusion protein Ag16113 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, heavy chain 10, non-muscle
Synonyms Myosin 10, NMMHC-B, NMMHC II-b, Myosin-10, Myosin heavy chain, non-muscle IIb
Calculated Molecular Weight 1985 aa, 230 kDa
Observed Molecular Weight 229 kDa
GenBank Accession NumberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_10804351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

实验方案

Product Specific Protocols
WB protocol for MYH10 antibody 21403-1-APDownload protocol
IF protocol for MYH10 antibody 21403-1-APDownload protocol
IP protocol for MYH10 antibody 21403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineWB,IP,IF

J Agric Food Chem

LncRNA BlncAD1 Modulates Bovine Adipogenesis by Binding to MYH10, PI3K/Akt Signaling Pathway, and miR-27a-5p/CDK6 Axis

Authors - Xin Li
View Article
humanWB,IP,IF

Nature

TMEFF1 is a neuron-specific restriction factor for herpes simplex virus

Authors - Yao Dai
View Article