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ABI2 Polyclonal antibody

ABI2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14890-1-AP

Print datasheet

Synonyms

Abelson interactor 2, ABI 2, ABI2, ABI2B, Abl binding protein 3, abl interactor 2, AblBP3, AIP 1, Arg binding protein 1, ArgBP1, argBPIA, argBPIB, SSH3BP2

验证数据展示

  • WB analysis using 14890-1-AP

    WB analysis using 14890-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14890-1-AP

    IHC staining of human brain using 14890-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 14890-1-AP

    IHC staining of human brain using 14890-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse heart tissue, mouse uterus tissue, mouse testis tissue, mouse brain tissue, HeLa cells, K-562 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14890-1-AP targets ABI2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ABI2 fusion protein Ag6679 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name abl interactor 2
Synonyms Abelson interactor 2, ABI 2, ABI2, ABI2B, Abl binding protein 3, abl interactor 2, AblBP3, AIP 1, Arg binding protein 1, ArgBP1, argBPIA, argBPIB, SSH3BP2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 52-56 kDa
GenBank Accession NumberBC001439
Gene Symbol ABI2
Gene ID (NCBI) 10152
RRIDAB_2288997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABI2 antibody 14890-1-APDownload protocol
IHC protocol for ABI2 antibody 14890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Nat Commun

GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis

Authors - Qiang Zhu
View Article
mouse,humanWB

Nat Commun

hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS)

Authors - Hannah E Salapa
View Article
humanWB

Nat Cell Biol

Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis

Authors - Xiaoguang Liu
View Article