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CoraLite® Plus 488-conjugated ACAT2 Monoclonal antibody

ACAT2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F5F11

Cat No : CL488-68005

Print datasheet

Synonyms

ACAT2, ACTL

验证数据展示

  • FC experiment of HepG2 using CL488-68005

    FC experiment of HepG2 using CL488-68005

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ACAT2 (CL488-68005, Clone:1F5F11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68005-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68005 targets ACAT2 in FC (Intra) applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat, rabbit
Immunogen ACAT2 fusion protein Ag6596 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acetyl-Coenzyme A acetyltransferase 2
Synonyms ACAT2, ACTL
Calculated Molecular Weight 41 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC000408
Gene Symbol ACAT2
Gene ID (NCBI) 39
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BWD1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ACAT2 antibody CL488-68005Download protocol
Standard Protocols
Click here to view our Standard Protocols