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- KD/KO Validated
APOL1 Polyclonal antibody
APOL1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, HepG2 cells, A2780 cells, human plasma, SW 1990 cells, A549 cells, SKOV-3 cells, HeLa cells, MCF-7 cells |
Positive IP detected in | HepG2 cells, human plasma tissue |
Positive IHC detected in | human kidney tissue, human liver tissue, human pancreas tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells, HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11486-2-AP targets APOL1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, Trypanosoma brucei |
Immunogen | APOL1 fusion protein Ag2016 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | apolipoprotein L, 1 |
Synonyms | APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 39 kDa |
GenBank Accession Number | BC017331 |
Gene Symbol | APOL1 |
Gene ID (NCBI) | 8542 |
RRID | AB_2058396 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14791 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for APOL1 antibody 11486-2-AP | Download protocol |
IHC protocol for APOL1 antibody 11486-2-AP | Download protocol |
IF protocol for APOL1 antibody 11486-2-AP | Download protocol |
IP protocol for APOL1 antibody 11486-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Exp Med Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model. | ||
Proc Natl Acad Sci U S A Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes. | ||
J Am Soc Nephrol Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.
| ||
J Am Soc Nephrol Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.
| ||
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. |