验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, HT-29 cells, HepG2 cells, mouse brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17247-1-AP targets ATPB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ATPB fusion protein Ag11177 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
| 别名 | EC:7.1.2.2, ATPSB, ATPMB, ATP5F1B, ATP5B |
| 计算分子量 | 529 aa, 57 kDa |
| 观测分子量 | 45-57 kDa |
| GenBank蛋白编号 | BC016512 |
| 基因名称 | ATPB |
| Gene ID (NCBI) | 506 |
| RRID | AB_2061878 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06576 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATPB antibody 17247-1-AP | Download protocol |
| IHC protocol for ATPB antibody 17247-1-AP | Download protocol |
| IF protocol for ATPB antibody 17247-1-AP | Download protocol |
| IP protocol for ATPB antibody 17247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Sci Adv Proteomimetic polymer blocks mitochondrial damage, rescues Huntington's neurons, and slows onset of neuropathology in vivo | ||
Nat Commun Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. | ||
Nat Commun ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models. | ||
Brain Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy. |
