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验证数据展示

  • IF Staining of HepG2 using CL488-66051

    IF Staining of HepG2 using CL488-66051

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated Albumin antibody (CL488-66051, Clone: 4A1C11 ) at dilution of 1:400.

  • FC experiment of HepG2 using CL488-66051

    FC experiment of HepG2 using CL488-66051

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Albumin (CL488-66051, Clone:4A1C11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66051

产品说明书

CloneNo. 4A1C11
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig and More (1)

应用

IF/ICC, FC (Intra) and More (1)

别名

ALB, Serum albumin, HSA, 4A1C11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66051-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-66051 targets Albumin in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, rat, pig
文献引用反应性mouse
免疫原 Albumin fusion protein Ag9885 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 albumin
别名 ALB, Serum albumin, HSA, 4A1C11
计算分子量 609 aa, 69 kDa
观测分子量 66 kDa
GenBank蛋白编号BC034023
基因名称 Albumin
Gene ID (NCBI) 213
RRIDAB_2883241
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02768
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Albumin antibody CL488-66051Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Mol Cell

WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy.

Authors - Sixian Qi
View Article