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CoraLite® Plus 488-conjugated Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-23660

Print datasheet

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3

验证数据展示

  • IF Staining of H9C2 using CL488-23660

    IF Staining of H9C2 using CL488-23660

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

  • IF Staining of C2C12 using CL488-23660

    IF Staining of C2C12 using CL488-23660

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

经过测试的应用

Positive IF detected inH9C2 cells, C2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 23660-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23660 targets Alpha Actin in IF applications and shows reactivity with mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity mouse, rat
Immunogen Alpha Actin fusion protein Ag19151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, alpha 1, skeletal muscle
Synonyms ACTA, ACTA1, ACTA1 skeletal Muscle Actin, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC012597
Gene Symbol ACTA1
Gene ID (NCBI) 58
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68133
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha Actin antibody CL488-23660Download protocol
Standard Protocols
Click here to view our Standard Protocols