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CoraLite® Plus 488-conjugated BSEP Monoclonal antibody

BSEP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C11D5

Cat No : CL488-67512

Print datasheet

Synonyms

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

验证数据展示

  • IF Staining of HeLa using CL488-67512

    IF Staining of HeLa using CL488-67512

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 BSEP antibody (CL488-67512, Clone: 3C11D5 ) at dilution of 1:200. Blue (DAPI).

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67512-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67512 targets BSEP in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen BSEP fusion protein Ag29135 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 11
Synonyms ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP
Calculated Molecular Weight 146 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberNM_003742
Gene Symbol ABCB11
Gene ID (NCBI) 8647
RRIDAB_2919487
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95342
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BSEP antibody CL488-67512Download protocol
Standard Protocols
Click here to view our Standard Protocols