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Beta Tubulin Recombinant antibody

Beta Tubulin Recombinant Antibody for FC, IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish and More (1)

Applications

FC, IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2O13

Cat No : 80713-1-RR

Print datasheet

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3

验证数据展示

  • WB analysis using 80713-1-RR

    WB analysis using 80713-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 80713-1-RR

    IHC staining of rat brain using 80713-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IF Staining of C2C12 using 80713-1-RR

    IF Staining of C2C12 using 80713-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of rat brain using 80713-1-RR

    IHC staining of rat brain using 80713-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IP experiment of HEK-293 using 80713-1-RR

    IP experiment of HEK-293 using 80713-1-RR

    IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:66240-1-Ig 1:10000) with HEK-293 cells lysate 1360 ug.

  • FC experiment of HEK-293 using 80713-1-RR

    FC experiment of HEK-293 using 80713-1-RR

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human Beta Tubulin (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, zebrafish tissue, HEK-293 cells, Jurkat cells, K-562 cells, Hsc-T6 cells, NIH/3T3 cells, mouse brain, rat brain
Positive IP detected inHEK-293 cells
Positive IF detected inC2C12 cells, HeLa cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

80713-1-RR targets Beta Tubulin in WB, IP, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Applications FC, IF/ICC, IP, WB, ELISA
Cited ApplicationsIF
Tested Reactivity Human, mouse, rat, zebrafish
Cited Reactivityhuman
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, MC1R, TUBB, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2918906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
IHC protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
IF protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
IP protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
FC protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cells

Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties

Authors - Hidetaka Togo
View Article