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C1R Polyclonal antibody

C1R Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17346-1-AP

Print datasheet

Synonyms

C1R, Complement C1r subcomponent

验证数据展示

  • WB analysis of human blood using 17346-1-AP

    WB analysis of human blood using 17346-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 17346-1-AP (C1R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17346-1-AP

    IHC staining of human liver using 17346-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17346-1-AP (C1R Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 17346-1-AP

    IHC staining of human liver using 17346-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17346-1-AP (C1R Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 17346-1-AP is C1R Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen C1R fusion protein Ag11390 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 1, r subcomponent
Synonyms C1R, Complement C1r subcomponent
Calculated Molecular Weight 705 aa, 80 kDa
Observed Molecular Weight 85 kDa, 30 kDa
GenBank Accession NumberBC035220
Gene Symbol C1R
Gene ID (NCBI) 715
RRIDAB_2878392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein which can be cleaved into an A chain and a B chain upon activation. This antibody recognizes full-length C1r chain and the cleaved B chain.

实验方案

Product Specific Protocols
WB protocol for C1R antibody 17346-1-APDownload protocol
IHC protocol for C1R antibody 17346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
View Article