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CoraLite® Plus 488-conjugated CD107a / LAMP1 Monoclonal antibody

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B2E9

Cat No : CL488-67300

Print datasheet

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

验证数据展示

  • IF Staining of human liver cancer using CL488-67300

    IF Staining of human liver cancer using CL488-67300

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 CD107a antibody (CL488-67300, Clone: 3B2E9 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL488-67300

    IF Staining of human liver cancer using CL488-67300

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 CD107a antibody (CL488-67300, Clone: 3B2E9 ) at dilution of 1:200.

  • FC experiment of NK92 using CL488-67300

    FC experiment of NK92 using CL488-67300

    1X10^6 NK92 were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD107a / LAMP1 (CL488-67300, Clone:3B2E9) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman liver cancer tissue
Positive FC detected inNK92

This antibody is not recommended for immunocytofluorescent assays.

Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67300-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67300 targets CD107a / LAMP1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Human
Immunogen CD107a / LAMP1 fusion protein Ag28869 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 90-120 kDa
GenBank Accession NumberBC021288
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_2919446
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11279
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-67300Download protocol
FC protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-67300Download protocol
Standard Protocols
Click here to view our Standard Protocols