验证数据展示
经过测试的应用
| Positive FC detected in | Human peripheral blood platelets |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
65185-1-Ig targets CD61 in IF, FC applications and shows reactivity with human, non-human primates samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, non-human primates |
| 文献引用反应性 | human |
| 免疫原 | N/A 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| 别名 | Integrin beta 3, ITGB3, VIPL2, Integrin beta-3, GPIIIa |
| 计算分子量 | 788 aa, 87 kDa |
| GenBank蛋白编号 | BC127666 |
| 基因名称 | Integrin beta 3 |
| Gene ID (NCBI) | 3690 |
| RRID | AB_2881484 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Purified by protein-A affinity chromatography |
| UNIPROT ID | P05106 |
| 储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD61 antibody 65185-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |