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CoraLite® Plus 488-conjugated CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H3A4

Cat No : CL488-67145

Print datasheet

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

验证数据展示

  • FC experiment of Jurkat using CL488-67145

    FC experiment of Jurkat using CL488-67145

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CDC25B (CL488-67145, Clone:1H3A4) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67145-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67145 targets CDC25B in FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Mouse, rat
Immunogen CDC25B fusion protein Ag27193 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cell division cycle 25 homolog B (S. pombe)
Synonyms CDC25B, CDC25HU2, M phase inducer phosphatase 2
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30305
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CDC25B antibody CL488-67145Download protocol
Standard Protocols
Click here to view our Standard Protocols