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CES1 Monoclonal antibody

CES1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C11C1

Cat No : 67079-1-Ig

Print datasheet

Synonyms

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

验证数据展示

  • WB analysis of U-937 using 67079-1-Ig

    WB analysis of U-937 using 67079-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 67079-1-Ig (CES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67079-1-Ig

    IHC staining of human liver using 67079-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CES1 antibody included
  • IHC staining of human liver using 67079-1-Ig

    IHC staining of human liver using 67079-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CES1 antibody included
  • IF Staining of HepG2 using 67079-1-Ig

    IF Staining of HepG2 using 67079-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67079-1-Ig (CES1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inU-937 cells, THP-1 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CES1 Ready-To-Use IHC Kit. CES1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67079-1-Ig targets CES1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen CES1 fusion protein Ag10647 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Synonyms ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase
Calculated Molecular Weight 566 aa, 62 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC012418
Gene Symbol CES1
Gene ID (NCBI) 1066
RRIDAB_2882387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP23141
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).

实验方案

Product Specific Protocols
WB protocol for CES1 antibody 67079-1-IgDownload protocol
IHC protocol for CES1 antibody 67079-1-IgDownload protocol
IF protocol for CES1 antibody 67079-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols