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CTSZ Polyclonal antibody

CTSZ Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16578-1-AP

Print datasheet

Synonyms

Cathepsin P, Cathepsin X, cathepsin Z, CTSX, CTSZ

验证数据展示

  • WB analysis using 16578-1-AP

    WB analysis using 16578-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16578-1-AP (CTSZ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16578-1-AP

    IP experiment of HeLa using 16578-1-AP

    IP result of anti-CTSZ (IP:16578-1-AP, 4ug; Detection:16578-1-AP 1:300) with HeLa cells lysate 2500ug.

  • IHC staining of human pancreas using 16578-1-AP

    IHC staining of human pancreas using 16578-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16578-1-AP (CTSZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 16578-1-AP

    IHC staining of human pancreas using 16578-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16578-1-AP (CTSZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16578-1-AP targets CTSZ in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CTSZ fusion protein Ag9865 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cathepsin Z
Synonyms Cathepsin P, Cathepsin X, cathepsin Z, CTSX, CTSZ
Calculated Molecular Weight 303 aa, 34 kDa
Observed Molecular Weight 34-37 kDa
GenBank Accession NumberBC042168
Gene Symbol CTSZ
Gene ID (NCBI) 1522
RRIDAB_2878282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CTSZ antibody 16578-1-APDownload protocol
IHC protocol for CTSZ antibody 16578-1-APDownload protocol
IP protocol for CTSZ antibody 16578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article