Claudin 1 Polyclonal antibody
Claudin 1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (6)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse skin tissue, rat skin tissue, HepG2 cells |
Positive IHC detected in | human skin cancer tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HUVEC cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28674-1-AP targets Claudin 1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse, rat, sheep, pig, canine |
Immunogen | Claudin 1 fusion protein Ag29269 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | claudin 1 |
Synonyms | claudin 1, CLD1, CLDN1, ILVASC, SEMP1 |
Calculated Molecular Weight | 211 aa, 23 kDa |
Observed Molecular Weight | 20-23 kDa |
GenBank Accession Number | BC012471 |
Gene Symbol | CLDN1 |
Gene ID (NCBI) | 9076 |
RRID | AB_2881190 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95832 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Claudin 1 antibody 28674-1-AP | Download protocol |
IHC protocol for Claudin 1 antibody 28674-1-AP | Download protocol |
IF protocol for Claudin 1 antibody 28674-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Microbiome Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1 | ||
J Nanobiotechnology Human umbilical cord mesenchymal stem cells derived exosome shuttling mir-129-5p attenuates inflammatory bowel disease by inhibiting ferroptosis | ||
Matrix Biol Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system | ||
Biomed Pharmacother Bacterial butyrate mediates the anti-atherosclerotic effect of silybin | ||
Biomed Pharmacother Polysaccharide-rich fractions from Enteromorpha prolifera improve hepatic steatosis and gut barrier integrity in high-fat diet-induced obese mice linking to modulation of gut microbiota | ||
Oxid Med Cell Longev Honokiol Ameliorates DSS-Induced Mouse Colitis by Inhibiting Inflammation and Oxidative Stress and Improving the Intestinal Barrier |