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CoraLite® Plus 488-conjugated Cytokeratin 5 Monoclonal antibody

Cytokeratin 5 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A1C5

Cat No : CL488-66727

Print datasheet

Synonyms

58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

验证数据展示

  • IF Staining of human oesophagus cancer using CL488-66727

    IF Staining of human oesophagus cancer using CL488-66727

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 488 Cytokeratin 5 antibody (CL488-66727, Clone: 1A1C5 ) at dilution of 1:200.

  • IF Staining of human oesophagus cancer using CL488-66727

    IF Staining of human oesophagus cancer using CL488-66727

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 488 Cytokeratin 5 antibody (CL488-66727, Clone: 1A1C5 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman oesophagus cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66727-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66727 targets Cytokeratin 5 in IF applications and shows reactivity with Human, mouse, pig samples.

Tested Applications IF-P
Tested Reactivity Human, mouse, pig
Immunogen Cytokeratin 5 fusion protein Ag24184 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 5
Synonyms 58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5
Calculated Molecular Weight 590 aa, 62 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC024292
Gene Symbol KRT5
Gene ID (NCBI) 3852
RRIDAB_2919361
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13647
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 5 antibody CL488-66727Download protocol
Standard Protocols
Click here to view our Standard Protocols