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CoraLite® Plus 488-conjugated FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C10G9

Cat No : CL488-67150

Print datasheet

Synonyms

FXYD1, Phospholemman, PLM

验证数据展示

  • FC experiment of C2C12 using CL488-67150

    FC experiment of C2C12 using CL488-67150

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FXYD1 (CL488-67150, Clone:2C10G9) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy FXYD1 Ready-To-Use IHC Kit. FXYD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67150-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67150 targets FXYD1 in FC (Intra) applications and shows reactivity with Human, Pig, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Pig, mouse
Immunogen FXYD1 fusion protein Ag25307 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name FXYD domain containing ion transport regulator 1
Synonyms FXYD1, Phospholemman, PLM
Calculated Molecular Weight 92 aa, 10 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC032800
Gene Symbol FXYD1
Gene ID (NCBI) 5348
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00168
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FXYD1 antibody CL488-67150Download protocol
Standard Protocols
Click here to view our Standard Protocols