CoraLite®594-conjugated GFP tag Monoclonal antibody
GFP tag Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein
Applications
WB, IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E10H7
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IF detected in | Transfected HEK-293 cells |
| Positive FC detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66002 targets GFP tag in WB, IF, FC (Intra) applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, IF/ICC, FC (Intra) |
| Tested Reactivity | recombinant protein |
| Immunogen | GFP tag fusion protein Ag2128 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GFP tag |
| Synonyms | CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, green, green fluorescence protein, green fluorescent protein, YFP |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | U73901 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2919937 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 594.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 GFP tag antibody CL594-66002 | Download protocol |
| IF protocol for CL594 GFP tag antibody CL594-66002 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |