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  • Featured Product
  • KD/KO Validated

GM2A Monoclonal antibody, PBS Only

GM2A Monoclonal Antibody for WB,FC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1E4D3

Cat No : 66080-1-PBS

Print datasheet

Synonyms

Ganglioside GM2 activator, GM2 AP, GM2 ganglioside activator, GM2A, SAP 3

验证数据展示

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

产品信息

66080-1-PBS targets GM2A in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications WB,FC,ELISA
Tested Reactivity human
Immunogen GM2A fusion protein Ag4394 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GM2 ganglioside activator
Synonyms Ganglioside GM2 activator, GM2 AP, GM2 ganglioside activator, GM2A, SAP 3
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17900
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.