验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60012 targets GRP94 in WB, IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | GRP94 fusion protein Ag1439 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heat shock protein 90kDa beta (Grp94), member 1 |
| 别名 | HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP |
| 计算分子量 | 96 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | BC009195 |
| 基因名称 | GRP94 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2919888 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P14625 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| IF protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| FC protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |