验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66387-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66387 targets GSDMD in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | GSDMD fusion protein Ag16827 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | gasdermin D |
| 别名 | DF5L, DFNA5L, FKSG10, FLJ12150, gasdermin D, GSDMD, GSDMDC1 |
| 计算分子量 | 484 aa, 53 kDa |
| 观测分子量 | 47-53 kDa |
| GenBank蛋白编号 | BC008904 |
| 基因名称 | GSDMD |
| Gene ID (NCBI) | 79792 |
| RRID | AB_2883325 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P57764 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GSDMD antibody CL488-66387 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |