CoraLite® Plus 647-conjugated Histone-H3 Polyclonal antibody
Histone-H3 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Histone-H3 Ready-To-Use IHC Kit. Histone-H3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17168-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL647-17168 is Histone-H3 Fusion Protein expressed in E. coli.
Tested Applications | FC (Intra) |
Tested Reactivity | human, mouse, rat |
Immunogen | Histone-H3 fusion protein Ag10644 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | histone cluster 2, H3a |
Synonyms | H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o |
Calculated Molecular Weight | 136 aa, 15 kDa |
Observed Molecular Weight | 15-17 kDa |
GenBank Accession Number | BC015544 |
Gene Symbol | HIST2H3A |
Gene ID (NCBI) | 333932 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q71DI3 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 Histone-H3 antibody CL647-17168 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |