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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ICAM-1 Monoclonal antibody

ICAM-1 Monoclonal Antibody for FC, IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F9A8

Cat No : CL488-60299

Print datasheet

Synonyms

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

验证数据展示

  • IF Staining of human lung cancer using CL488-60299

    IF Staining of human lung cancer using CL488-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CL488-60299 (ICAM-1 antibody) at dilution of 1:100.

  • IF Staining of human lung cancer using CL488-60299

    IF Staining of human lung cancer using CL488-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CL488-60299 (ICAM-1 antibody) at dilution of 1:100.

  • FC experiment of Raji using CL488-60299

    FC experiment of Raji using CL488-60299

    1X10^6 Raji cells were surface stained with 0.8 ug CoraLite® Plus 488 Anti-Human ICAM-1 (CL488-60299, Clone:2F9A8) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were not fixed.

经过测试的应用

Positive IF detected inhuman lung cancer tissue
Positive FC detected inRaji cells
For other applications, we recommend the unconjugated version of this antibody, 60299-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60299 targets ICAM-1 in IF, FC applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC
Tested Reactivity human
Immunogen ICAM-1 fusion protein Ag8309 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_2883137
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05362
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.

  1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

  2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

  3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

  4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

  5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ICAM-1 antibody CL488-60299Download protocol
FC protocol for CL Plus 488 ICAM-1 antibody CL488-60299Download protocol
Standard Protocols
Click here to view our Standard Protocols