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IGFBP3 Monoclonal antibody

IGFBP3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A11

Cat No : 68402-1-Ig

Print datasheet

Synonyms

BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3

验证数据展示

  • WB analysis of human placenta using 68402-1-Ig

    WB analysis of human placenta using 68402-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68402-1-Ig

    WB analysis using 68402-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 68402-1-Ig

    IHC staining of human placenta using 68402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68402-1-Ig (IGFBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IGFBP3 antibody included

经过测试的应用

Positive WB detected inA549 cells, human placenta tissue, Caco-2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IGFBP3 Ready-To-Use IHC Kit. IGFBP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68402-1-Ig targets IGFBP3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen IGFBP3 fusion protein Ag20351 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name insulin-like growth factor binding protein 3
Synonyms BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight39-45 kDa
GenBank Accession NumberBC000013
Gene Symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP17936
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

实验方案

Product Specific Protocols
WB protocol for IGFBP3 antibody 68402-1-IgDownload protocol
IHC protocol for IGFBP3 antibody 68402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols