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CoraLite® Plus 488-conjugated IMP3 Monoclonal antibody

IMP3 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E7D6

Cat No : CL488-66247

Print datasheet

Synonyms

BRMS2, C15orf12, DKFZp586L0118, FLJ10968, IMP3, MRPS4, U3 snoRNP protein IMP3

验证数据展示

  • FC experiment of HepG2 using CL488-66247

    FC experiment of HepG2 using CL488-66247

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IMP3 (CL488-66247, Clone:1E7D6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL488-66247

    IF Staining of HepG2 using CL488-66247

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated IMP3 antibody (CL488-66247, Clone: 1E7D6 ) at dilution of 1:100, CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66247-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66247 targets IMP3 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen IMP3 fusion protein Ag23602 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Synonyms BRMS2, C15orf12, DKFZp586L0118, FLJ10968, IMP3, MRPS4, U3 snoRNP protein IMP3
Calculated Molecular Weight 184 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC020557
Gene Symbol IMP3
Gene ID (NCBI) 55272
RRIDAB_2883293
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NV31
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004]. CoraLite488-Conjugated IMP3 antibody can stain green. Ex: 488nm, Em: 515nm

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IMP3 antibody CL488-66247Download protocol
FC protocol for CL Plus 488 IMP3 antibody CL488-66247Download protocol
Standard Protocols
Click here to view our Standard Protocols