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验证数据展示

  • FC experiment of HepG2 using CL488-66247

    FC experiment of HepG2 using CL488-66247

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IMP3 (CL488-66247, Clone:1E7D6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL488-66247

    IF Staining of HepG2 using CL488-66247

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated IMP3 antibody (CL488-66247, Clone: 1E7D6 ) at dilution of 1:100, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated IMP3 Monoclonal antibody

IMP3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66247

产品说明书

CloneNo. 1E7D6

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66247-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66247 targets IMP3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 IMP3 fusion protein Ag23602 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
别名 MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6
计算分子量 184 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC020557
基因名称 IMP3
Gene ID (NCBI) 55272
RRIDAB_2883293
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NV31
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004]. CoraLite488-Conjugated IMP3 antibody can stain green. Ex: 488nm, Em: 515nm

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IMP3 antibody CL488-66247Download protocol
FC protocol for CL Plus 488 IMP3 antibody CL488-66247Download protocol
Standard Protocols
Click here to view our Standard Protocols