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ISOC2 Polyclonal antibody

ISOC2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27082-1-AP

Print datasheet

Synonyms

ISOC2

验证数据展示

  • WB analysis using 27082-1-AP

    WB analysis using 27082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27082-1-AP (ISOC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27082-1-AP

    IHC staining of human kidney using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 27082-1-AP

    IHC staining of human kidney using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inLNCaP cells, mouse kidney tissue, mouse liver tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27082-1-AP targets ISOC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ISOC2 fusion protein Ag23936 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name isochorismatase domain containing 2
Synonyms ISOC2
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017344
Gene Symbol ISOC2
Gene ID (NCBI) 79763
RRIDAB_2880748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AB3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ISOC2 antibody 27082-1-APDownload protocol
IHC protocol for ISOC2 antibody 27082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols