Cytokeratin 15 Polyclonal antibody
Cytokeratin 15 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
FC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse thymus tissue, HeLa cells, PC-3 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human cervical cancer tissue, human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy KRT15 Ready-To-Use IHC Kit. KRT15 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10137-1-AP targets Cytokeratin 15 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Cytokeratin 15 fusion protein Ag0185 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | keratin 15 |
| Synonyms | CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC002641 |
| Gene Symbol | KRT15 |
| Gene ID (NCBI) | 3866 |
| RRID | AB_2234359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19012 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 15 antibody 10137-1-AP | Download protocol |
| IHC protocol for Cytokeratin 15 antibody 10137-1-AP | Download protocol |
| IP protocol for Cytokeratin 15 antibody 10137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomater Sci A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring | ||
Carcinogenesis Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein. | ||
Sci Rep Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers. | ||
Front Med (Lausanne) Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells. |
