LACTB Monoclonal antibody
LACTB Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
IF, IHC, WB,ELISA
Conjugate
Unconjugated
CloneNo.
3C3C3
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66785-1-Ig targets LACTB in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF, IHC, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | LACTB fusion protein Ag12233 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lactamase, beta |
| Synonyms | FLJ14902, G24, lactamase, beta, LACTB, MRPL56 |
| Calculated Molecular Weight | 547 aa, 61 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC067288 |
| Gene Symbol | LACTB |
| Gene ID (NCBI) | 114294 |
| RRID | AB_2882130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P83111 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LACTB is also named as MRPL56,whic is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LACTB antibody 66785-1-Ig | Download protocol |
| IHC protocol for LACTB antibody 66785-1-Ig | Download protocol |
| IF protocol for LACTB antibody 66785-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.
| ||
Cancer Manag Res MicroRNA-1276 Promotes Colon Cancer Cell Proliferation by Negatively Regulating LACTB. | ||
Mol Cell OXCT1 functions as a succinyltransferase, contributing to hepatocellular carcinoma via succinylating LACTB |