CoraLite® Plus 488-conjugated LACTB Monoclonal antibody
LACTB Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C3C3
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66785-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66785 targets LACTB in IF applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | Human |
| Immunogen | LACTB fusion protein Ag12233 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lactamase, beta |
| Synonyms | FLJ14902, G24, lactamase, beta, LACTB, MRPL56 |
| Calculated Molecular Weight | 547 aa, 61 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC067288 |
| Gene Symbol | LACTB |
| Gene ID (NCBI) | 114294 |
| RRID | AB_2919368 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P83111 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LACTB is also named as MRPL56,whic is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LACTB antibody CL488-66785 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |