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LBP Monoclonal antibody

LBP Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A7E3

Cat No : 66181-1-Ig

Print datasheet

Synonyms

LBP

验证数据展示

  • WB analysis of Jurkat using 66181-1-Ig

    WB analysis of Jurkat using 66181-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66181-1-Ig (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66181-1-Ig

    IHC staining of human liver cancer using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LBP antibody included
  • IHC staining of human liver cancer using 66181-1-Ig

    IHC staining of human liver cancer using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LBP antibody included

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LBP Ready-To-Use IHC Kit. LBP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66181-1-Ig targets LBP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen LBP fusion protein Ag13485 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lipopolysaccharide binding protein
Synonyms LBP
Calculated Molecular Weight 481 aa, 53 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC022256
Gene Symbol LBP
Gene ID (NCBI) 3929
RRIDAB_2881576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

实验方案

Product Specific Protocols
WB protocol for LBP antibody 66181-1-IgDownload protocol
IHC protocol for LBP antibody 66181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols