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CoraLite® Plus 488-conjugated MAP1B Monoclonal antibody

MAP1B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E6B9

Cat No : CL488-67423

Print datasheet

Synonyms

DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP 1B, MAP1B, MAP5

验证数据展示

  • FC experiment of SH-SY5Y using CL488-67423

    FC experiment of SH-SY5Y using CL488-67423

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MAP1B (CL488-67423, Clone:1E6B9) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 67423-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67423 targets MAP1B in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity Human, rat, mouse
Immunogen MAP1B fusion protein Ag17709 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name microtubule-associated protein 1B
Synonyms DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP 1B, MAP1B, MAP5
Calculated Molecular Weight 2468 aa, 271 kDa
GenBank Accession NumberBC141853
Gene Symbol MAP1B
Gene ID (NCBI) 4131
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46821
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MAP1B antibody CL488-67423Download protocol
Standard Protocols
Click here to view our Standard Protocols