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- KD/KO Validated
MGEA5 Polyclonal antibody
MGEA5 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | SMMC-7721 cells, BxPC-3 cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human pancreas tissue, human meningioma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14711-1-AP targets MGEA5 in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, RIP, IHC, CoIP, ChIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish, pig, Drosophila |
Immunogen | MGEA5 fusion protein Ag6405 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | meningioma expressed antigen 5 (hyaluronidase) |
Synonyms | Beta N acetylhexosaminidase, Bifunctional protein NCOAT, HAT, HEXC, Hexosaminidase C, KIAA0679, MEA5, Meningioma expressed antigen 5, MGEA5, N acetyl beta glucosaminidase, NCOAT, O GlcNAcase, OGA |
Calculated Molecular Weight | 103 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC039583 |
Gene Symbol | MGEA5 |
Gene ID (NCBI) | 10724 |
RRID | AB_2143063 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60502 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for MGEA5 antibody 14711-1-AP | Download protocol |
IHC protocol for MGEA5 antibody 14711-1-AP | Download protocol |
IF protocol for MGEA5 antibody 14711-1-AP | Download protocol |
IP protocol for MGEA5 antibody 14711-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
J Clin Invest Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation | ||
Biomaterials PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy | ||
Redox Biol Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. | ||
Proc Natl Acad Sci U S A Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells. | ||
Proc Natl Acad Sci U S A O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development. |