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  • Featured Product
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MME,CD10 Monoclonal antibody

MME,CD10 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B9D9

Cat No : 60034-3-Ig

Print datasheet

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

验证数据展示

  • FC experiment of Ramos using 60034-3-Ig

    FC experiment of Ramos using 60034-3-Ig

    1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (60034-3-Ig, Clone:4B9D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HEK-293 using 60034-3-Ig

    WB analysis of HEK-293 using 60034-3-Ig

    WB result of MME,CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MME,CD10 transfected HEK-293 cells.

  • WB analysis of pig kidney using 60034-3-Ig

    WB analysis of pig kidney using 60034-3-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 60034-3-Ig

    IHC staining of human renal cell carcinoma using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human renal cell carcinoma using 60034-3-Ig

    IHC staining of human renal cell carcinoma using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included

经过测试的应用

Positive WB detected inpig kidney tissue, mouse kidney tissue, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells, Raji cells
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60034-3-Ig targets MME,CD10 in WB, FC, IHC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity human, pig, mouse
Immunogen MME,CD10 fusion protein Ag0427 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name membrane metallo-endopeptidase
Synonyms Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11
Calculated Molecular Weight 750 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC101658
Gene Symbol MME
Gene ID (NCBI) 4311
RRIDAB_10641991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08473
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

实验方案

Product Specific Protocols
WB protocol for MME,CD10 antibody 60034-3-IgDownload protocol
IHC protocol for MME,CD10 antibody 60034-3-IgDownload protocol
FC protocol for MME,CD10 antibody 60034-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols