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CoraLite®594-conjugated MTAP Monoclonal antibody

MTAP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B1G6

Cat No : CL594-66706

Print datasheet

Synonyms

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

验证数据展示

  • IF Staining of HeLa using CL594-66706

    IF Staining of HeLa using CL594-66706

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66706 (MTAP antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66706-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66706 targets MTAP in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen MTAP fusion protein Ag17954 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylthioadenosine phosphorylase
Synonyms c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase
Calculated Molecular Weight 283 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
RRIDAB_2883617
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13126
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

实验方案

Product Specific Protocols
IF protocol for CL594 MTAP antibody CL594-66706Download protocol
Standard Protocols
Click here to view our Standard Protocols