• Featured Product
  • KD/KO Validated

MUC16,CA125 Polyclonal antibody

MUC16,CA125 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20077-1-AP

Print datasheet

Synonyms

CA 125, CA125, MUC 16, MUC16, MUC16,CA125, Mucin 16



经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20077-1-AP targets MUC16,CA125 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 16, cell surface associated
Synonyms CA 125, CA125, MUC 16, MUC16, MUC16,CA125, Mucin 16
Calculated Molecular Weight 1519 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberNM_024690
Gene Symbol MUC16
Gene ID (NCBI) 94025
RRIDAB_10695048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXI7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.

实验方案

Product Specific Protocols
WB protocol for MUC16,CA125 antibody 20077-1-APDownload protocol
IHC protocol for MUC16,CA125 antibody 20077-1-APDownload protocol
IP protocol for MUC16,CA125 antibody 20077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Sci

Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells.

Authors - Tomoaki Furuta
humanWB

Toxicology

Necroptosis contributes to airborne particulate matter-induced ocular surface injury.

Authors - Kexin Shi
humanWB

Int J Oncol

Lewis antigen‑negative pancreatic cancer: An aggressive subgroup.

Authors - Chen Liu
humanWB

Biochem Biophys Res Commun

MUC16 mutation predicts a favorable clinical outcome and correlates decreased Warburg effect in gastric cancer.

Authors - Huijin Zhao
humanWB

J Exp Clin Cancer Res

Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer.

Authors - Zengqi Tan
  • KD Validated
humanWB

Cancers (Basel)

Protein Panel of Serum-Derived Small Extracellular Vesicles for the Screening and Diagnosis of Epithelial Ovarian Cancer

Authors - Huiling Lai