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NEDD4L Monoclonal antibody

NEDD4L Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F6H6

Cat No : 67276-1-Ig

Print datasheet

Synonyms

FLJ33870, hNedd4 2, KIAA0439, NEDD4 2, NEDD4.2, NEDD4L, NEDL3, RSP5

验证数据展示

  • WB analysis of Jurkat using 67276-1-Ig

    WB analysis of Jurkat using 67276-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 67276-1-Ig

    IF Staining of PC-3 using 67276-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunofluorescence (IF)IF : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67276-1-Ig targets NEDD4L in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen NEDD4L fusion protein Ag4565 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Synonyms FLJ33870, hNedd4 2, KIAA0439, NEDD4 2, NEDD4.2, NEDD4L, NEDL3, RSP5
Calculated Molecular Weight 854 aa, 96 kDa
Observed Molecular Weight 112 kDa, 125 kDa
GenBank Accession NumberBC032597
Gene Symbol NEDD4L
Gene ID (NCBI) 23327
RRIDAB_2882545
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96PU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

实验方案

Product Specific Protocols
WB protocol for NEDD4L antibody 67276-1-IgDownload protocol
IF protocol for NEDD4L antibody 67276-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols