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PGM1 Polyclonal antibody
PGM1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse skin tissue, HEK-293 cells, Jurkat cells, mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15161-1-AP targets PGM1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PGM1 fusion protein Ag7269 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphoglucomutase 1 |
| Synonyms | Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC001756 |
| Gene Symbol | PGM1 |
| Gene ID (NCBI) | 5236 |
| RRID | AB_2161415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36871 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PGM1 antibody 15161-1-AP | Download protocol |
| IHC protocol for PGM1 antibody 15161-1-AP | Download protocol |
| IF protocol for PGM1 antibody 15161-1-AP | Download protocol |
| IP protocol for PGM1 antibody 15161-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.
| ||
Brain C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis. | ||
J Hazard Mater Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes. | ||
Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. | ||
Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma | ||
iScience MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes. |
