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- KD/KO Validated
CoraLite® Plus 488-conjugated PINK1 Polyclonal antibody
PINK1 Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | mouse brain tissue |
For other applications, we recommend the unconjugated version of this antibody, 23274-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-23274 targets PINK1 in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P |
| Tested Reactivity | human, mouse, rat |
| Immunogen | PINK1 fusion protein Ag19825 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PTEN induced putative kinase 1 |
| Synonyms | BRPK, FLJ27236, PARK6, PINK1, PTEN induced putative kinase 1 |
| Calculated Molecular Weight | 581 aa, 63 kDa |
| Observed Molecular Weight | 65 kDa, 45 kDa |
| GenBank Accession Number | BC028215 |
| Gene Symbol | PINK1 |
| Gene ID (NCBI) | 65018 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXM7 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PINK1 antibody CL488-23274 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |