beta Actin Antibody 133 Publications

Mouse Monoclonal| Catalog number: 66009-1-Ig |CloneNo.: 2D4H5

Featured Product KD/KO validated

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规格

单价

100 μl

¥980

500 μl

¥4000

1000 μl

¥6000


Species specificity:
human, mouse, rat, hamster, zebrafish, monkey, goat, pig

Positive WB detected in:
multi-cells, A549 cells, HeLa

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue, human kidney tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:20-1:2000
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Fusion Protein

Full name:
actin, beta

Calculated molecular weight:

Observed molecular weight:
42 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ACTB

Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin
Background

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b.


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