验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66886 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | S6 Ribosomal protein fusion protein Ag6599 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | ribosomal protein S6 |
Synonyms | 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6 |
Calculated Molecular Weight | 29 kDa |
Observed Molecular Weight | 29-32 kDa |
GenBank Accession Number | BC000524 |
Gene Symbol | RPS6 |
Gene ID (NCBI) | 6194 |
RRID | AB_2883632 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P62753 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 S6 Ribosomal protein antibody CL594-66886 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |