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SF1 Polyclonal antibody

SF1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11635-1-AP

Print datasheet

Synonyms

BBP, D11S636, mBBP, SF1, splicing factor 1, Transcription factor ZFM1, ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, ZNF162

验证数据展示

  • WB analysis using 11635-1-AP

    WB analysis using 11635-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 11635-1-AP

    IHC staining of mouse ovary using 11635-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF1 antibody included
  • IHC staining of mouse ovary using 11635-1-AP

    IHC staining of mouse ovary using 11635-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SF1 Ready-To-Use IHC Kit. SF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11635-1-AP targets SF1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SF1 fusion protein Ag2209 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 1
Synonyms BBP, D11S636, mBBP, SF1, splicing factor 1, Transcription factor ZFM1, ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, ZNF162
Calculated Molecular Weight 638 aa, 68 kDa
Observed Molecular Weight65-71 kDa
GenBank Accession NumberBC020217
Gene Symbol SF1
Gene ID (NCBI) 7536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.

实验方案

Product Specific Protocols
WB protocol for SF1 antibody 11635-1-APDownload protocol
IHC protocol for SF1 antibody 11635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Retrovirology

A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression.

Authors - Rebecca Moschall
View Article
humanIHC,IF

Cancers (Basel)

Targeting Oncogenic Wnt/β-Catenin Signaling in Adrenocortical Carcinoma Disrupts ECM Expression and Impairs Tumor Growth

Authors - Morgan K Penny
View Article